Last edited by Maulkis
Monday, April 20, 2020 | History

7 edition of Bacterial Artificial Chromosomes: Volume 1 found in the catalog.

Bacterial Artificial Chromosomes: Volume 1

Library Construction, Physical Mapping, and Sequencing (Methods in Molecular Biology)

by

  • 22 Want to read
  • 11 Currently reading

Published by Humana Press .
Written in English

    Subjects:
  • Molecular biology,
  • Scientific equipment & techniques, laboratory equipment,
  • Life Sciences - Bacteriology,
  • Chromosomes,
  • Science,
  • Bacterial Genetics,
  • Laboratory manuals,
  • Science/Mathematics,
  • Cloning, Molecular,
  • Chromosomes, Artificial, Bacte,
  • Biotechnology,
  • Life Sciences - Genetics & Genomics,
  • Science / Microbiology,
  • Bacterial artificial chromosom,
  • Bacterial artificial chromosomes,
  • Chromosome Mapping,
  • Chromosomes, Artificial, Bacterial

  • Edition Notes

    ContributionsShaying Zhao (Editor), Marvin Stodolsky (Editor)
    The Physical Object
    FormatHardcover
    Number of Pages384
    ID Numbers
    Open LibraryOL8253803M
    ISBN 100896039889
    ISBN 109780896039889


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Bacterial Artificial Chromosomes: Volume 1 Download PDF EPUB FB2

Buy Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing (Methods in Molecular Biology) on FREE SHIPPING on qualified orders Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing (Methods in Molecular Biology): Zhao, Shaying, Stodolsky, Marvin: :.

In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs.

Editors: Narayanan, Kumaran (Ed.) Usually dispatched within 3 to 5 business days. Usually dispatched within 3 to 5 business days. Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with BACs.

This book focuses on the numerous applications of Bacterial Artificial Chromosomes (BACs) in a variety of studies. The topics reviewed range from using BAC libraries as resources for marsupial and monotreme gene mapping and comparative genomic studies, to using BACs as vehicles for Bacterial Artificial Chromosomes: Volume 1 book the large infectious DNA genomes of by: 2.

Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing (Methods in Molecular Biology). Lecture 1 12/6/ BAC vectors (Bacterial Artificial Chromosome) BAC vectors Bacterial Artificial Chromosomes: Volume 1 book plasmids constructed with the replication origin of E.

coli F factor, and so can be maintained in a single copy per cell. These vectors can hold DNA fragments of up to kb.

Since they are present in low copies, recombination between the high-copy plasmids. Bacterial Artificial Chromosomes: Volume 2 Functional Studies Michael M. Weil, Rashmi Pershad, Ruoping Wang (auth.), Shaying Zhao, Marvin Stodolsky (eds.) The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine.

Boysen, C, Simon, M., and Hood, L. () Analysis of the Mb human α/δ T-cell receptor locus with bacterial artificial chromosome clones. Genome Res. 7, – PubMed Google ScholarCited by: Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with d chapters Bacterial Artificial Chromosomes: Volume 1 book in Bacterial Artificial Chromosomes: Volume 1 book edition present fundamental techniques used for BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model.

Get this from a library. Bacterial artificial chromosomes. [Shaying Zhao; Marvin Stodolsky;] -- Describes the methods for transferring and maintaining DNA within and between species, presented in step-by-step format. Includes scientific reviews, Bacterial Artificial Chromosomes: Volume 1 book tools, database resources and sequencing.

Expression in adult sagittal sections Bacterial Artificial Chromosomes: Volume 1 book a BAC transgenic line prepared using BAC RPF19 (a) or BAC RPD19 (b). Panels 1–6 show higher-magnification images of the cerebral cortex (panel 1), basal forebrain (panels 2 and Cited by: Several developmental and historical threads are woven and displayed in these two volumes of Bacterial Artificial Chromosomes, the first on Library Construction, Physical Mapping, and Sequencing, and the second on Fu- tional Studies.

The use of large-insert clone libraries is the unifying feature. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.\/span>\"@ en\/a> ; \u00A0\u00A0\u00A0\n schema:description\/a> \" BAC.

Free 2-day shipping. Buy Methods in Molecular Biology: Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing (Hardcover) at Marjorie A. Hoy, in Insect Molecular Genetics (Third Edition), BACs.

Bacterial artificial chromosomes (BACs) were developed so that scientists could insert very large segments of DNA and have it cloned in E. can incorporate an average of kb of exogenous DNA and are based on the E. coli F-factor plasmid; the E.

coli F-factor plasmid maintains a low copy number in. Find many great new & used options and get the best deals for Methods in Molecular Biology: Bacterial Artificial Chromosomes Vol. 1: Library Construction, Physical Mapping, and Sequencing (, Hardcover) at the best online prices at eBay.

Free shipping for many products. An accompanying volume, Volume 1: Library Construction, Physical Mapping, and Sequencing, presents readily reproducible techniques for library construction, physical mapping, and sequencing.

Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection Format: Hardcover. Bacterial Artificial Chromosomes: Volume 2: Functional Studies (Methods in Molecular Biology) by Shaying Zhao, Marvin Stodolsky. Humana Press.

Used - Very Good. Former Library book. Great condition for a used book. Minimal wear. The Oryza Bacterial Artificial Chromosome Library Resource: Construction and Analysis of 12 Deep-Coverage Large-Insert BAC Libraries That Represent the 10 Genomes Types of the Genus Oryza.

Genome Research. Shaying Zhao is the author of Bacterial Artificial Chromosomes ( avg rating, 1 rating, 0 reviews, published ) and Bacterial Artificial Chromosome 4/5(1).

Bacterial Artificial Chromosomes: Volume 1: Library Construction Physical Mapping and Sequencing. Bacterial Artificial Chromosome (BAC) libraries are a valuable research resource. Any one of the clones in these libraries can carry hundreds of thousands of base pairs of genetic information.

Often the entire coding sequence and significant upstream and downstream regions, including regulatory elements, can be found in a single BAC by: 1. / Site-specific integration of bacterial artificial chromosomes into human cells. Bacterial Artificial Chromosomes.

editor / Kumaran Narayanan. Vol. 2nd. NY USA: Springer, pp. (Methods in Molecular Biology).Author: Bradley McColl, Bradley McColl, Sara Howden, Sara Howden, Jim Vadolas, Jim Vadolas. Bacterial pathogens have been becoming the main problem in hospital and community-acquired infections.

It is hard to treat the strains that are resistant to antibiotics, due to the causing recurrent and untreatable infections. In recent years, the combination treatments and the novel technologies have been preferred to overcome the emergence of antibacterial resistance of pathogens.

In this Author: Sahra. (source: Nielsen Book Data) Summary Bacterial Artificial Chromosomes provides a comprehensive collection of the protocols and resources developed for BACs in recent years.

These two volumes collectively cover four topics about BACs: 1) library construction, 2) physical mapping, 3) sequencing, and 4) functional studies. Bacterial strains and plasmids used in this study are listed in Table 1.

coli and B. subtilis strains were routinely grown in Luria-Bertani broth (LB). For generating competent B. subtilis, cells were cultivated in the starvation medium as described needed, growth media were supplemented with ampicillin ( μg/ml), kanamycin (50 μg/ml) and chloramphenicol (30 μg/ml) for Cited by: 7.

Zhao S and Stodolsky M () Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Se-quencing (Methods in Molecular Biology).

Totowa, NJ: Humana : Dave Ussery. Almazán F, González JM, Pénzes Z, et al. Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome. Proc Natl Acad Sci U S A. ; – doi: /pnas [PMC free article] [Google Scholar]Cited by: The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine.

In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs.

Bring the volume of the Xba I-digested, MBN-treated sample up to µl with dH 2O. Add an equal volume of phenol:chloroform:isoamyl alcohol () to the diluted sample, vortex vigorously for 1 min, spin at 13, × g for 10 min, and then transfer the Cited by: 2.

Replication-Competent Bacterial Artificial Chromosomes of Marek's Disease Virus: Novel Tools for Generation of Molecularly Defined Herpesvirus Vaccines Lawrence Petherbridge, 1 Ken Howes, 1 Susan J.

Baigent, 1 Melanie A. Sacco, 1 Simon Evans, 1 Nikolaus Osterrieder, 2, 3 and Venugopal Nair 1, *Cited by: Bacterial artificial chromosomes (BACs) were constructed containing full-length, infectious DNA of HSV-1 strains 17 and KOS.

To generate BACs without altering viral genes, sequences required for selection and propagation of the BAC were placed between the U L 37 and U L 38 genes, and flanked by LoxP sites. The system was tested by studying multiple properties of these HSV-1 BAC constructs in Cited by:   This study aimed to exploit bacterial artificial chromosomes (BAC) as large antigen-capacity DNA vaccines (BAC-VAC) against complex pathogens, such as herpes simplex virus 1 (HSV-1).

The kbp HSV-1 genome recently has been cloned as an F-plasmid-based BAC in Escherichia coli (fHSV), which can efficiently produce infectious virus progeny upon Cited by: We constructed representative large-insert bacterial artificial chromosome (BAC) libraries of two human pathogens (Trypanosoma brucei and Giardia lamblia) using a new hybrid vector, pTARBAC1, containing a yeast artificial chromosome (YAC) cassette (a yeast selectable marker and a centromere).The cassette allows transferring of BACs into yeast for their further by: VolumeIssues 1–2, SeptemberPages Short communication.

Rapid generation of markerless recombinant MVA vaccines by en passant recombineering of a self-excising bacterial artificial chromosome. Author links open overlay panel Matthew G. Cottingham Sarah C. by: Cutting-edge and much-needed, Mammalian Artificial Chromosomes: Methods and Protocols offers a timely collection of meticulous techniques that will help elucidate the mechanisms of nuclear duplication, allow the introduction of changes into the human genome, and revolutionize our understanding of how chromosomes work.

A loxP-transposon retrofitting strategy for generating large nested deletions from one end of the insert DNA in bacterial artificial chromosomes and P1 artificial chromosomes was described recently [Chatterjee, P.

& Coren, J. () Nucleic Acids Res. 25, –].In this report, we combine this procedure with direct sequencing of nested-deletion templates by using primers located Cited by: Cyanobacteria are photosynthetic microorganisms that inhabit almost all geographic locations, including oceans, caves, ponds, lakes, soil, snow, and hot springs.

Their acumen to survive and succeed in a plethora of ambiance is due to their extraordinary metabolism and adaptations. Their wide applications include therapeutics, cosmeceuticals, wastewater remediation, biofuels, antioxidative. BAC (bacterial artificial chromosome) 大腸菌を宿主とする人工染色体ベクター。大腸菌プラスミドの一種Fプラスミドの複製系を利用する。最大約 kbの断片を安定にクローン化することができる。 PAC (P1-derived artificial chromosome).

Lack of reliable techniques for chromosome identification is the major obstacle for cytogenetics research in plant species with large numbers of small chromosomes.

To promote molecular cytogenetics research of potato (Solanum tuberosum, 2n = 4x = 48) we developed a bacterial artificial chromosome (BAC) library of a diploid potato species by:. Get this from a library! Pdf artificial chromosomes. Volume 2, Functional studies.

[Shaying Zhao; Marvin Stodolsky;] -- The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine.

In Bacterial Artificial Chromosomes.New drugs download pdf frequently entering into the market along with the existing drugs.

The antibacterial agents can be discussed in five major classes, i.e. classification based on the type of action, source, spectrum of activity, chemical structure and function.

Resistance of bacteria to antibiotics is an urgent problem of the humanity, which leads us to the lack of therapy for serious bacterial.A Search for Ebook Agents Edited by Varaprasad Bobbarala This book contains precisely referenced chapters, emphasizing antibacterial agents with clinical practicality and alternatives to synthetic antibacterial agents through detailed reviews of Cited by: